In year 2004, while working in Gas Authority of India Limited (GAIL (India) Limited) in a remote location in Gujrat, I faced a near fatal cardiovascular problem. I somehow survived and came to my hometown, New Delhi. I applied for a transfer to New Delhi which was denied by GAIL’s management and I was asked to pay bribe if I wanted a transfer to New Delhi. As I was always interested in research for which there was no scope in an operating company like GAIL and because of my medical condition, I decided to leave GAIL. I started developing a chemical process simulation software in year 2004 itself using my limited knowledge of MS excel and limited knowledge of chemical engineering principles gathered while performing day to day plant activities in GAIL. Side by side, I started looking for job within or near Delhi. I could not find a job suiting to my medical condition and finally in year 2006, I opened up a small company for trading survey equipments with my savings from GAIL and taking loan on my parental property. Meanwhile I gathered knowledge about MS excel and chemical engineering principles from Internet and few standard books and applied this knowledge to develop my chemical engineering simulator. I launched a website to distribute results of my software for nominal charges in year 2008 and started developing a technology for desalinating water using gas hydrate concept again by using my already gathered knowledge and skills and gathering knowledge about gas hydrates and other new concepts again from Internet and other readily available books and articles. In year 2010, I started developing a flow assurance software along with the chemical process simulation software which was reasonably developed by that time. In year 2012, I started working on horizontal distillation technology that gave a horizontal alternative to vertical tray columns which are used in refineries and other chemical plants and are generally 40-50 feet high requiring unsafe, difficult and expensive aerial jobs while transportation, installation, operations, maintenance and troubleshooting. I kept on filing provisional applications, PCT applications and patents for my technologies as per requirement. I recently finished working on my third technology and have filed a PCT application for the technology. My complete research work (not including my third technology) is showcased on VLE, bubble point, dew point software website. I primarily worked independently and all alone for the past 13 years while developing my technologies and softwares. I am hopeful of getting US patent for two of my technologies in this year and am getting excellent reviews about my research work from various experts of the field. Though my trading company is in losses at present and I have not been able to generate much revenue from my research at present but I am hopeful of good monetary gains once I have granted US patents for my technologies.
There are many difficulties applying this technique to DNA, but is it possible (see Atomic Force Microscopy with Nanoscale Cantilevers Resolves Different Structural Conformations of the DNA Double Helix). First, the tips used in these experiments are also made of atoms and have a radius of curvature of about 10nm, which is comparable to the size of the DNA. If you want to see atomic level detail, you need a probe which is atomically sized as well, or else the atomic detail will be blurred out by the interactions of many atoms at once. In addition, it is difficult to prepare samples, align the probe on the uneven surface of DNA, etc. I believe the second image, of the DNA, comes from transmission electron microscopy (TEM). This consists of basically shooting electrons through the DNA and detecting them on the other side. The first problem is that to see atomic detail, it is necessary to use high energy electrons, which tends to quickly damage any biomolecules, and the small signal size from a single strand of DNA requires a lot of electrons. The best TEM images are actually of cross sections of slices of inorganic crystals, where many layers of the slice of the crystal contribute to the image, which results in less damage. Second, the small signal from DNA has to be separated from whatever is holding the DNA. This may require suspending the DNA, which adds further complications. The structure of DNA was originally solved with diffraction experiments, where a crystal of DNA is analyzed to get atomic level detail, but direct imaging of single molecules remains very challenging.